nuclear staining with dapi (Beyotime)
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nuclear staining with dapi
Nuclear Staining With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 34692 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuclear+staining+with+dapi/DAPI/pm42791620-89-34-39
Average 99 stars, based on 34692 article reviews
Nuclear Staining With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 34692 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuclear+staining+with+dapi/DAPI/pm42791620-89-34-39
Average 99 stars, based on 34692 article reviews
nuclear staining with dapi - by Bioz Stars,
2026-11
99/100 stars
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Blocking Assay:Article Title: Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease Article Snippet: .. Subsequent steps included serum blocking, incubation with a primary antibody (Affinity Biosciences Cat# AF0016), thorough washing, and Incubation:Article Title: Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease Article Snippet: .. Subsequent steps included serum blocking, incubation with a primary antibody (Affinity Biosciences Cat# AF0016), thorough washing, and Article Title: From liver to lens: How low-intensity ultrasound targets diabetic cataract mechanisms. Article Snippet: Background: Diabetic cataract is a common complication of diabetes, and current clinical management mainly relies on surgical intervention, with limited preventive strategies available.. This study aimed to investigate whether liver-targeted low-intensity pulsed ultrasound (LIPUS) can prevent or delay the progression of diabetic cataract and to elucidate the underlying molecular mechanisms.. Methods: A type 2 diabetes mellitus rat model was established using a high-fat diet combined with streptozotocin. Article Title: Role of eCIRP in Mediating Post-ischemia Microglial Phagocytosis via TREM-2 Receptor: Insights from Porcine and Mouse Cellular Models. Article Snippet: Cerebral ischemia/reperfusion (I/R) injury triggers potent neuroinflammatory responses, in which microglial activation and dysregulated phagocytosis may contribute to neuronal damage.. Extracellular cold-inducible RNA-binding protein (eCIRP), a damage-associated molecular pattern, has been implicated in postischemic inflammation; however, its role in regulating microglial phagocytosis remains poorly understood.. This study employed a porcine model of cardiac arrest/reperfusion (CA/R) and a mouse cellular model of oxygen–glucose deprivation/reoxygenation (OGD/R) in BV2 microglia and N2a neuron to investigate the role of the eCIRP–TREM2 signaling in microglial activation. Staining:Article Title: Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease Article Snippet: .. Subsequent steps included serum blocking, incubation with a primary antibody (Affinity Biosciences Cat# AF0016), thorough washing, and Article Title: Hepatocyte Growth Hormone Receptor Ablation Is Associated With Aging Phenotypes and Hepatic Mitochondrial Dysfunction. Article Snippet: Oligomycin (1.5 μM), FCCP (1.0 μM), and rotenone/antimycin A (0.5 μM) were sequentially injected, and key parameters, including non- mitochondrial oxygen consumption, basal respiration, maximal respiration, ATP production, and spare respiratory capacity, were analyzed using Wave software (Agilent). .. After reaching about 70% confluence in confocal dishes, cells were stained with Mito- Tracker (1 mM, C1997S, Beyotime Biotechnology, China) or BODIPY 493/503 (1 μg/mL, C2053S, Beyotime Biotechnology, China) for 30 min, followed by Article Title: TRIM59 alleviates neuronal ferroptosis and promotes functional recovery after spinal cord injury by mediating ubiquitination and degradation of ANXA2 Article Snippet: .. Subsequently, the sections received secondary antibodies (Alexa Fluor 488 or Alexa Fluor 594 conjugates; 1:200; Shanghai Fushen Biotechnology) for 60 min at ambient temperature, then underwent Article Title: From liver to lens: How low-intensity ultrasound targets diabetic cataract mechanisms. Article Snippet: Background: Diabetic cataract is a common complication of diabetes, and current clinical management mainly relies on surgical intervention, with limited preventive strategies available.. This study aimed to investigate whether liver-targeted low-intensity pulsed ultrasound (LIPUS) can prevent or delay the progression of diabetic cataract and to elucidate the underlying molecular mechanisms.. Methods: A type 2 diabetes mellitus rat model was established using a high-fat diet combined with streptozotocin. Article Title: Borneol-grafted poly(amidoamine) dendrimer with dual antimicrobial and enamel-remineralizing activities against early-stage dental caries. Article Snippet: For cytotoxicity screening, HOKs were exposed to BN-PAMAM (0.5–4 mg/mL) for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology (Beyotime), Shanghai, China) at 450 nm. .. For comparative testing, HOKs were treated with BN-PAMAM, PAMAM, or BN (2 mg/ mL), or controls, for 24 h. Live/Dead staining (Thermo Fisher), F-actin staining with phalloidin (Beyotime), and Confocal:Article Title: Hepatocyte Growth Hormone Receptor Ablation Is Associated With Aging Phenotypes and Hepatic Mitochondrial Dysfunction. Article Snippet: Oligomycin (1.5 μM), FCCP (1.0 μM), and rotenone/antimycin A (0.5 μM) were sequentially injected, and key parameters, including non- mitochondrial oxygen consumption, basal respiration, maximal respiration, ATP production, and spare respiratory capacity, were analyzed using Wave software (Agilent). .. After reaching about 70% confluence in confocal dishes, cells were stained with Mito- Tracker (1 mM, C1997S, Beyotime Biotechnology, China) or BODIPY 493/503 (1 μg/mL, C2053S, Beyotime Biotechnology, China) for 30 min, followed by Fluorescence:Article Title: Borneol-grafted poly(amidoamine) dendrimer with dual antimicrobial and enamel-remineralizing activities against early-stage dental caries. Article Snippet: For cytotoxicity screening, HOKs were exposed to BN-PAMAM (0.5–4 mg/mL) for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology (Beyotime), Shanghai, China) at 450 nm. .. For comparative testing, HOKs were treated with BN-PAMAM, PAMAM, or BN (2 mg/ mL), or controls, for 24 h. Live/Dead staining (Thermo Fisher), F-actin staining with phalloidin (Beyotime), and Microscopy:Article Title: Borneol-grafted poly(amidoamine) dendrimer with dual antimicrobial and enamel-remineralizing activities against early-stage dental caries. Article Snippet: For cytotoxicity screening, HOKs were exposed to BN-PAMAM (0.5–4 mg/mL) for 24 h. Cell viability was evaluated using a Cell Counting Kit-8 (CCK-8; Beyotime Biotechnology (Beyotime), Shanghai, China) at 450 nm. .. For comparative testing, HOKs were treated with BN-PAMAM, PAMAM, or BN (2 mg/ mL), or controls, for 24 h. Live/Dead staining (Thermo Fisher), F-actin staining with phalloidin (Beyotime), and |